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DNA ligases seal 5′-PO4 and three′-OH polynucleotide ends by way of a few nucleotidyl transfer measures involving ligase-adenylate and DNA-adenylate intermediates. DNA ligases are crucial guardians of genomic integrity, and ligase dysfunction underlies human genetic condition syndromes. Crystal constructions of DNA ligases certain to nucleotide and nucleic acid substrates have illuminated how ligase response chemistry is catalyzed, how ligases recognize weakened DNA finishes, And exactly how protein domain movements and Energetic-site reworking are utilized to choreograph the tip-joining pathway.

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